|主要用途||小型全基因组测序 组合试剂 试剂盒||应用领域||医疗卫生,化工,生物产业,制药|
Illumina 二代测序组合试剂盒 FC-121-9999
"Illumina/TruSeq FFPE DNA Library Prep QC Kit (24 samples)/FC-121-9999/1 Ea
" Illumina 产品编号： FC-121-9999美 元 价： $155.00会 员 价： 待定品 牌： Illumina产 地： 美国公 司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:
The TruSeq Custom Amplicon Low Input Library Prep Kit is a scalable amplicon sequencing solution that delivers sensitive and specific results from both low-input and formalin-fixed, paraffin-embedded (FFPE) DNA samples. It offers:
Accurate variant detection from as low as 10 ng of input genomic DNA (gDNA) and challenging FFPE samples
Fully supported, optimized workflow solution including simple data analysis
Automation-friendly workflow that can be completed in 6 hours, with only 3 hours of hands-on time
Low DNA Input and FFPE Compatibility
The TruSeq Custom Amplicon Low Input Library Prep Kit is a fully customizable, amplicon-based assay for targeted resequencing starting from as low as 10 ng of genomic DNA (gDNA). This scalable assay allows researchers to capture multiple targets of interest simultaneously and sequence up to 1536 amplicons in a single pool, using a single reaction. This library prep kit offers the flexibility to accommodate FFPE samples, such as preserved tumor tissue.
Confident Assay Design
Design targeted TruSeq Custom Amplicon oligonucleotide probes with DesignStudio, a free, easy-to-use online tool that provides optimized coverage.
Learn more about DesignStudio
Start a project in DesignStudio now (login required)
Assess FFPE Sample Quality before Sequencing
The TruSeq FFPE DNA Library Prep QC Kit uses a simple qPCR reaction to determine DNA quality and provide guidance on sequencing parameters. This step ensures that only samples that will achieve the necessary sequencing metrics are prepared, conserving resources that might be used on potentially low-quality, unrecoverable samples. The results of the QC step determine the recommended amount of input DNA. The TruSeq FFPE DNA Library Prep QC Kit can be bundled with the TruSeq Custom Amplicon Low Input Library Prep Kit to maximize lab budgets.
For FFPE applications, we suggest designing your project with 150 bp or 175 bp amplicons since FFPE DNA is highly degraded. TruSeq Custom Amplicon Low Input cannot be used with applications requiring 425 bp amplicons.
The TruSeq Custom Amplicon Low Input Library Prep Kit supports human, mouse, rat, and bovine samples. DesignStudio design algorithms are also available for maize, rice, pig, dog, soybean, chicken, and sheep; however, assay performance cannot be guaranteed as the resulting assays have not been tested. In addition, Illumina Concierge services can design custom assays and/or provide assay optimization support for any species of interest.
Inquire about Illumina Concierge services
Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit
Assay Time 1 day
Hands-On Time 4 hours
Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality
Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.
Multiplexing Up to 384 samples per sequencing run
Mechanism of Action Amplification
TruSeq Custom Amplicon Library Prep Protocol:
The TruSeq Custom Amplicon assay is a simple and streamlined method for capturing and amplifying targeted regions of interest.
Seamless Amplicon Sequencing Workflow:
The TruSeq Custom Amplicon Low Input Library Prep Kit is part of an integrated, fully supported workflow for amplicon sequencing that guides researchers from design through data analysis. Illumina technical and field specialists help ensure rapid resolution and minimize potential laboratory downtime.
Robust Variant Detection at Low DNA Input:
The TruSeq Custom Amplicon Low Input Library Prep Kit demonstrates high concordance between expected and observed variant frequencies for 10 ng high-quality FFPE DNA. Reference DNA samples were prepared following the TruSeq Custom Amplicon Low Input workflow with the TruSeq Amplicon - Cancer Panel primer pool, and sequenced on the MiSeq System in replicates of 4. Variants were called using MiSeq Reporter Software. R2 values are shown."
Sequencing Kits/ Microarray Kits/Informatics Products/ Clinical Research Products/In Vitro Diagnostic/ Products/Molecular Biology Reagents/ Accessory Products
咨询illumina MiSeq测序试剂盒等产品欢迎您致电 火博体育(中国)搜狗百科：1850 1609 238张经理
Illumina 二代测序组合试剂盒 FC-121-9999